- The 14 November 2003 draft of the screenplay credits Brian Helgeland for a rewrite. He is not credited in the final film.
- The taxi that Bourne drives during the car chase is a Russian-made Volga 3110.
- When Bourne calls Pamela Landy from the rooftop, a voice is heard in her office saying that they "need 90 seconds to triangulate his position". Bourne disconnects the call exactly 88 seconds later.
- Unlike the James Bond franchise, all the devices that Bourne uses are real and can be purchased by the average citizen.
- The pen Jason Bourne uses to jot his phone booth notes is a Rotring 600. This is a moderately expensive ballpoint ($30-40).
- The phone that Jason Bourne uses is a Siemens ME45.
- The piano music heard in the lobby of the Hotel Brecker (where Bourne killed Neski) is Frédéric Chopin's Ballade no. 4 in F minor.
- The first scene shot was the scene in Moscow where Bourne speaks to a taxi driver and arranges to pay in Dollars.
- The film originally ended with the confession to Neski's daughter. Following previews, which found the ending too bleak, the New York postscript scene with Bourne and Landy was shot, just weeks before the film's release in the summer of 2004.
- Virtually all of the events in the movie were shot in the reverse order of location. This means scenes in Moscow were shot first and those in Goa were shot last
- In the house in Munich, when Jason Bourne uses the rolled newspaper as a weapon, the martial art he performs is derived from Escrima, an old Philippine martial art, also called Arnis or Kali. This fighting style mainly uses sticks to fight, and in modern times the use of objects from our everyday life is taught, including ball pens (as seen in The Bourne Identity (2002)) and rolled up newspapers.
- The gun Bourne uses in Moscow is Sig Sauer PRO. Kirill uses a Walther P99 with a QPQ finished slide through the whole movie. QPQ stands for Quench, Polish, Quench. This is a finishing process that results in a silver-colored slide.
- Visible body count: 9
- The first location of shooting was scheduled for the Kievski Vokzal (Kiev Train Station). However, it was scheduled for demolition, which commenced a matter of days before the arrival of the film crew. According to some news reports, they had to reconstruct some of the station to achieve a convincing looking set.
- The average length of a shot is 1.9 seconds.
- To give this movie its gritty, documentary-style appearance, director Paul Greengrass used mostly handheld cameras, and a muted color palette. Greengrass also made sure to avoid computer graphics at all costs, and all of the stunts shown in the movie were achieved practically.
- Matt Damon accidentally knocked out Tim Griffin who played the CIA interrogator John Nevins during the scene in the consulate when Bourne takes him and a security guard down after being captured.
- Unlike the first movie, screenwriter Tony Gilroy did read the book this time and claimed that he did a re-imagination not adaptation of the novel. What he meant that was he wrote an original screenplay from scratch using key events / characters from the novel as framework except discarding the traditional Carlos The Jackal-type villain for Kiril
- As with the previous film. The martial arts used by Jason Bourne and others is a combination of Filipino Kali and Bruce Lee's Jeet Kune Do.
- Producer Frank Marshall selected Paul Greengrass as director after he'd seen Greengrass's Bloody Sunday (2002). Marshall was after a director who wasn't intrinsically associated with the action genre, feeling that Greengrass would impart an original spin of his own to the script.
- Jason Bourne never smiles in the movie.
- The film was made with no intention of making a third movie after this one; the final scene was also meant to give the Bourne character some closure and properly end the series. When the third film was green-lit, the writers had to write the story around this epilogue.
>>> WARNING: Here Be Spoilers <<<
Trivia items below here contain information that may give away important plot points. You may not want to read any further if you've not already seen this title.
- SPOILERS: Before Brian Helgeland did his rewrite, Tony Gilroy's initial draft of the screenplay was vastly different. Instead of Kirill shooting her, Marie dies by accident when a bus veers off the road and slams into her. Bourne is outraged and goes berserk on the driver, almost killing him until the police arrest him. A large section of the film is then spent in an Indian prison, where Bourne makes numerous allies and enemies before planning his escape. From then on in, both scripts follow a similar track.
- SPOILER: It took 4 days to shoot the sequence where three assassins close in on a house they suspect harbors Bourne, but he has already left and rigged the house to explode. When it does, three stuntmen wearing cabled harnesses are violently yanked up and away from the explosion, landing on off-camera airbags. Six cameras simultaneously covered the the brief sequence.
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