- Production was delayed for over a month while Charlize Theron recuperated from a neck injury she sustained while slipping during stunt training. After the injury she still insisted on doing her own stunts, although she does have a stunt doubles clause in her contracts.
- Michelle Rodriguez was originally set to play the lead when the film was announced in 2003.
- Charlize Theron had trampoline training with Cirque du Soleil's Terry Bartlett.
- WILHELM SCREAM: A guard is shot and falls off a stone wall in the courtyard gun battle toward the end of the movie.
- The scene where Aeon catches a fly with her eyelash is taken directly from the anime TV show.
- The production team first chose the Brazilian capital of Brasilia as the city in which to shoot the movie. However, because of logistical problems they ended up in Berlin, Germany, where they found most of their locations, such as a recently built crematorium and underground sewers dating from before World War II.
- Theatrical trailer contains alternate/unused footage (ie. Aeon wears a less revealing set of clothes when she gets up).
- When Aeon is leaping over the lethal grass and lands balanced on a corner, the overhead shot closely resembles the logo from the "Aeon Flux" (1991) series.
- Famke Janssen turned down the lead role.
- At one point, Darren Aronofsky was attached to direct this film.
- The white knife Una uses to chop vegetables in her kitchen is made by Kyocera electronics; the blade is made of a patented ceramic material instead of steel.
>>> WARNING: Here Be Spoilers <<<
Trivia items below here contain information that may give away important plot points. You may not want to read any further if you've not already seen this title.
- SPOILER: In the original anime, Aeon was killed at the end of each episode, only to appear alive and well at the beginning of the next. In the film, all the people in the world have died and been re-born over and over in a most subtle reference/adaptation of the original series.
- SPOILER: The flower Cladius is handling when Aeon comes and confronts him about Una's death is a Rafflesia, and smells like rotten meat. Its local name translates into "corpse flower" and it is commonly found in southeast Asia. It is the official flower for Sabah, Malaysia.
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