- Continuity: When Reaper sees 'Lucy' in the laboratory, his earpiece switches sides from right ear to left ear when the shot changes from his close up to showing him and his sister.
- Audio/visual unsynchronized: When Mac Takahashi (Yao Chin) is decapitated, Sarge (The Rock) demands a response from him. If the English subtitles are activated, the Sarge's dialogue is titled as "Mac".
- Factual errors: When Sarge and Destroyer confront and kill a monkey hiding in a section of AC conduit in front of them, they fire their weapons from approximately a 50 degree angle (i.e. if the floor is "6PM" then the ducting is at about "10PM). However, when the targeted ducting is shown, the bullet holes are perfectly parallel to the floor. Something that could have only happened had they shot from directly the beneath the air duct.
- Factual errors: When Duke and Samantha are talking while she's examining the creature (right before he goes to get the power bone saw), he corrects her by saying the Marine term is 'Hooah,' when in fact it's 'Ooh rah'. 'Hooah' is an Army term, not a Marine term.
- Factual errors: Throughout the movie, Sarge calls the Marines "soldiers." In reality, Marines are deeply offended when they are called "soldiers."
- Revealing mistakes: When Sarge punches through the glass sign during the finale, pre-cut lines (making it easier to punch through the glass) are visible on its surface.
- Continuity: In the bathroom stall just before Portman dies, the screen shows what he is seeing. The view of the gun on the right side of the screen, suggesting it is being held in a right handed manner, Portman however holds his weapon left handed.
- Revealing mistakes: In the scene in which The Kid asks Portman for the "Game Face" Pills: when Portman drops the pills into Kid's hand, all but the 2 that drop out are glued securely inside the bottle.
- Incorrectly regarded as goofs: When Portman and Goat check the chemical levels in the airlock, Portman mentions magnesium, chromium, and lead. We see his "chemical detection" device, and in the order he stated, follow the symbols Mg, Cr, and Ld. The symbol for lead is not Ld, but Pb. However, this is not necessarily a goof, since the device is not labeled as a Periodic Table, and the display may be "dumbed down" for common usage by lay people. "Ld" is a reasonable abbreviation for Lead for people without a chemistry or Latin background (Latin for metallic lead: Plumbum).
>>> WARNING: Here Be Spoilers <<<
Goofs below here contain information that may give away important plot points. You may not want to read any further if you've not already seen this title.
- Continuity: SPOILER: In the scene where Goat comes back to "life", the wounds and blood on his face/neck switch sides.
- Audio/visual unsynchronized: SPOILER: When Goat is smashing his head against the window and Sam's reflection is seen, her mouth is not moving but she is heard screaming.
- Crew or equipment visible: SPOILER: When Sarge makes his first jump up onto the stairwell in the final fight with Grimm, the two wires which lift him are visible.
- Revealing mistakes: SPOILER: Goat's eyes open and close between the time he dies in the sewer to when he is seen lying in the med-lab.
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